Core–shell structured phosphorescent nanoparticles for detection of exogenous and endogenous hypochlorite in live cells via ratiometric imaging and photoluminescence lifetime imaging microscopy† †Electronic supplementary information (ESI) available: Experimental procedures, characterisation data, Scheme S1 and Fig. S1–S3. See DOI: 10.1039/c4sc02600d

Core-shell structured phosphorescent nanoparticles for detection of exogenous and endogenous hypochlorite in live cells via ratiometric imaging and photoluminescence lifetime imaging microscopy.

We report a ratiometric phosphorescence sensory system for hypochlorite (ClO-) based on core-shell structured silica nanoparticles. Two phosphorescent iridium(iii) complexes were immobilised in the inner solid core and outer mesoporous layer of the nanoparticles, respectively. The former is insensitive to ClO- and thus serves as an internal standard to increase the accuracy and precision, while...

Development of a cyclometalated iridium complex with specific intramolecular hydrogen-bonding that acts as a fluorescent marker for the endoplasmic reticulum and causes photoinduced cell death† †Electronic supplementary information (ESI) available: Detailed synthesis; photophysical data (Table S1); pH dependent phosphorescence lifetime of complex C2 (Table S2); crystallographic parameters of C2; selected bond distances and angles of C2 (Table S3); cyclic voltammetric data of complexes C1–C11 (Table S4); 1H NMR spectra of ligands and complexes (Fig. S1 and S3); ESI-MS spectra of ligands and complexes (Fig. S2 and S4); fluorescence spectra of the complexes in acetonitrile and at pH 4, 7 and 9, exponential decay curve of C2 (Fig. S5); pH dependent fluorescence spectrum of complexes C1–C11 (Fig. S6); DIC and confocal fluorescence images of live MCF7 cells not treated with C2 but exposed to photoirradiation at 405 nm for 30 min; the cells were treated with DCFDA and fluorescence images were obtained at 529 nm after excitation at 495 nm (Fig. S7). ESI videos 1 and 2. CCDC 967841. For ESI and crystallographic data in CIF or other electronic format see DOI: 10.1039/c4dt00845f Click here for additional data file. Click here for additional data file. Click here for additional data file. Click here for additional data file. Click here for additional data file.

Cyclometalated iridium complexes have important applications as phosphorescent probes for cellular imaging due to their photophysical properties. Moreover, these properties also make them potential candidates as photosensitizers for photodynamic therapy (PDT) of tumors and skin diseases. Treatment of MCF7 breast carcinoma cells with a heteroleptic phosphorescent cyclometalated iridium(III) comp...

A DNA-based parity generator/checker for error detection through data transmission with visual readout and an output-correction function† †Electronic supplementary information (ESI) available: Scheme S1, Tables S1–S3 and Fig. S1–S11. See DOI: 10.1039/c6sc04056j Click here for additional data file.

Photoresponsive immunomagnetic nanocarrier for capture and release of rare circulating tumor cells† †Electronic supplementary information (ESI) available: Detailed experimental procedures and materials; Fig. S1–S8 and Tables S1–S3. See DOI: 10.1039/c5sc01380a Click here for additional data file.

Ultrahigh affinity Raman probe for targeted live cell imaging of prostate cancer† †Electronic supplementary information (ESI) available: Fig. S1–S7. See DOI: 10.1039/c6sc01739h Click here for additional data file.